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The cells were then scraped on ice, transferred to pre-chilled microcentrifuge tubes, centrifuged at 15,000 g for 20 min and the supernatants were collected

Control medium samples were generated by incubating the treatment medium in cell-free 6-well plates under the same conditions and duration as the cell culture samples

It's a, it's a whole process and your body needs to be clean this whole time

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Lifestyle, oxidative stress, and antioxidants: back and forth in the pathophysiology of chronic diseases